GCGR Recombinant antibody

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  WB analysis using 84237-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84237-5-RR (GCGR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84237-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84237-5-RR against Human GCGR were performed. The affinity constant is 71.6 pM.

GCGR {ptg:Brand} Recombinant Antibody for WB, ELISA

Cat No. 84237-5-RR

Clone No.241501C4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

glucagon receptor, GL-R, GL R, GGR, 241501C4

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Tested Applications

• Positive WB detected in: mouse heart tissue, mouse kidney tissue, mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84237-5-RR targets GCGR in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GCGR fusion protein Ag24861
• Full Name: glucagon receptor
• Calculated Molecular Weight: 477 aa, 54 kDa
• Observed Molecular Weight: 62-68 kDa
• GenBank Accession Number: BC104854
• Gene Symbol: GCGR
• Gene ID (NCBI): 2642
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P47871
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glucagon receptor (GCGR) is a secretin-like (class B) family of G-protein coupled receptors (GPCRs). It plays an important role in elevating the glucose concentration in the blood and has thus become one of the promising therapeutic targets for treating type 2 diabetes mellitus (PMID: 26236379). GCGR is a 62-kDa glycoprotein that contains at least four N-linked oligosaccharide chains (PMID: 2174441; 15245877). Defects in the gene of GCGR cause non-insulin-dependent diabetes mellitus (NIDDM).