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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated GBX2 Polyclonal antibody

GBX2 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-21639

Synonyms

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated GBX2 Polyclonal anti (CL488-21639)

    IF Staining of HeLa using CL488-21639

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 GBX2 antibody (CL488-21639) at dilution of 1:200.

Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-21639 targets GBX2 in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GBX2 fusion protein Ag16288
Full Name gastrulation brain homeobox 2
Calculated Molecular Weight 348 aa, 37 kDa
Observed Molecular Weight 33-37 kDa
GenBank Accession NumberBC137448
Gene Symbol GBX2
Gene ID (NCBI) 2637
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Homeobox protein GBX-2 (GBX-2) is a homeobox gene essential for normal development of the midbrain and the anterior hindbrain. It mainly expressed in the brain, and located in nucleoplasm. It is presumably a transcription factor that is likely to regulate the expression of genes involved in establishing early A/P pattern in the neural plate (PMID: 9247335).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 GBX2 antibody CL488-21639Download protocol
Standard Protocols
Click here to view our Standard Protocols