GBP1 Recombinant antibody

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  WB analysis using 83751-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83751-6-RR (GBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 83751-6-RR

  1x10^6 HeLa cells untreated (dashed lines) or treated with IFN gamma (red) were intracellularly stained with 0.25 ug GBP1 Recombinant antibody (83751-6-RR, Clone: 240634G8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83751-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83751-6-RR against Human GBP1 were performed. The affinity constant is below 1 pM.

GBP1 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 83751-6-RR

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

Guanine nucleotide-binding protein 1, GTP-binding protein 1, GBP-1, GBP 1, EC:3.6.1.-

Formulation:  PBS and Azide

PBS and Azide

PBS Only

Conjugate:  Unconjugated

Unconjugated

Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

• Positive WB detected in: A2780 cells, HUVEC cells
• Positive FC (Intra) detected in: IFN gamma (100ng/ml 24h) HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83751-6-RR targets GBP1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GBP1 fusion protein Ag7562
• Full Name: GTP binding protein 1
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC002666
• Gene Symbol: GBP1
• Gene ID (NCBI): 2633
• RRID: AB_3671347
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P32455
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Guanylate binding protein 1 (GBP1) belongs to the dynamin superfamily of large GTPases. The expression of GBP1 is induced by interferon and GBP1 is characterized by its ability to specifically bind guanine nucleotides such as GMP, GDP, and GTP and its ability to hydrolyze GTP to GDP and GMP. GBP1 is induced in response to type I and type II interferons and as such plays a role in protective immunity against a spectrum of intracellular pathogens ranging from viruses to bacteria to protozoa, such as negative-strand RNA Rhabdovirus, vesicular stomatitis virus, and the positive-strand RNA Picornovirus, encephalomyocarditis virus in cultured cells, the inhibition of Chlamydia trachomatis, Toxoplasma gondii, and Salmonella enterica.