GBP1 Monoclonal antibody, PBS Only

GBP1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, pig, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A10E8

Cat no : 67161-1-PBS

Synonyms

GBP 1, GBP1, GTP binding protein 1, HuGBP 1

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Validation Data Gallery

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  WB analysis of HeLa using 67161-1-Ig (same clone as 67161-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67161-1-Ig (GBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67161-1-PBS in a different storage buffer formulation.

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  WB analysis of HUVEC using 67161-1-Ig (same clone as 67161-1-PBS)

  HUVEC cells were subjected to SDS PAGE followed by western blot with 67161-1-Ig (GBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67161-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 67161-1-Ig (same clone as 67161-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67161-1-Ig (GBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67161-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 67161-1-Ig (same clone as 67161-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67161-1-Ig (GBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67161-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig spleen using 67161-1-Ig (same clone as 67161-1-PBS)

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 67161-1-Ig (GBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67161-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat spleen using 67161-1-Ig (same clone as 67161-1-PBS)

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 67161-1-Ig (GBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67161-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 67161-1-Ig (same clone as 67161-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67161-1-Ig (GBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67161-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 67161-1-Ig (same clone as 67161-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67161-1-Ig (GBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67161-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67161-1-PBS targets GBP1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, pig, rat samples.

• Tested Reactivity: Human, pig, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: GBP1 fusion protein Ag7767
• Full Name: GTP binding protein 1
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC002666
• Gene Symbol: GBP1
• Gene ID (NCBI): 2633
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Guanylate binding protein 1 (GBP1) belongs to the dynamin superfamily of large GTPases. The expression of GBP1 is induced by IFN and GBP1 is characterized by its ability to specifically bind guanine nucleotides such as GMP, GDP, and GTP and its ability to hydrolyze GTP to GDP and GMP. GBP1 is induced in response to type I and type II IFNs and as such plays a role in protective immunity against a spectrum of intracellular pathogens ranging from viruses to bacteria to protozoa, such as negative-strand RNA Rhabdovirus, vesicular stomatitis virus and the positive-strand RNA Picornovirus, encephalomyocarditis virus in cultured cells, the inhibition of Chlamydia trachomatis, Toxoplasma gondii, and Salmonella enterica.