GBP1 Monoclonal antibody

GBP1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, pig, rat and More (3)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

3

CloneNo.

2A10E8

Cat no : 67161-1-Ig

Synonyms

GBP 1, GBP1, GTP binding protein 1, HuGBP 1

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Validation Data Gallery

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  WB analysis of HeLa using 67161-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67161-1-Ig (GBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HUVEC using 67161-1-Ig

  HUVEC cells were subjected to SDS PAGE followed by western blot with 67161-1-Ig (GBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67161-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67161-1-Ig (GBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 67161-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67161-1-Ig (GBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig spleen using 67161-1-Ig

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 67161-1-Ig (GBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 67161-1-Ig

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 67161-1-Ig (GBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 67161-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67161-1-Ig (GBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 67161-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67161-1-Ig (GBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, human placenta tissue, HUVEC cells, pig spleen tissue, rat spleen tissue, HEK-293 cells
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 2 publications below

Product Information

67161-1-Ig targets GBP1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, pig, rat samples.

• Tested Reactivity: Human, pig, rat
• Cited Reactivity: human, mouse, Chinese tree shrews
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: GBP1 fusion protein Ag7767
• Full Name: GTP binding protein 1
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC002666
• Gene Symbol: GBP1
• Gene ID (NCBI): 2633
• RRID: AB_2882457
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Guanylate binding protein 1 (GBP1) belongs to the dynamin superfamily of large GTPases. The expression of GBP1 is induced by IFN and GBP1 is characterized by its ability to specifically bind guanine nucleotides such as GMP, GDP, and GTP and its ability to hydrolyze GTP to GDP and GMP. GBP1 is induced in response to type I and type II IFNs and as such plays a role in protective immunity against a spectrum of intracellular pathogens ranging from viruses to bacteria to protozoa, such as negative-strand RNA Rhabdovirus, vesicular stomatitis virus and the positive-strand RNA Picornovirus, encephalomyocarditis virus in cultured cells, the inhibition of Chlamydia trachomatis, Toxoplasma gondii, and Salmonella enterica.

Publications

Species	Application	Title
human	WB,IF	Front Immunol; GBP1 Facilitates Indoleamine 2,3-Dioxygenase Extracellular Secretion to Promote the Malignant Progression of Lung Cancer.; Authors - Yinnan Meng
Chinese tree shrews	WB,IF	J Immunol; Tupaia GBP1 Interacts with STING to Initiate Autophagy and Restrict Herpes Simplex Virus Type 1 Infection.; Authors - Tianle Gu
mouse	WB	Vet Microbiol; Insights into the effect of guanylate-binding protein 1 on the survival of Brucella intracellularly; Authors - Zhiqiang Li ; KD Validated