GATM Monoclonal antibody, PBS Only

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  WB analysis of pig kidney using 68513-1-Ig (same clone as 68513-1-PBS)

  pig kidney tissue were subjected to SDS PAGE followed by western blot with 68513-1-Ig (GATM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68513-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68513-1-Ig (same clone as 68513-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68513-1-Ig (GATM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68513-1-PBS in a different storage buffer formulation.

GATM Monoclonal Antibody for WB, Indirect ELISA

Cat No. 68513-1-PBS

Clone No.1D12G5

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, Indirect ELISA

Synonyms

Transamidinase, L-arginine:glycine amidinotransferase, EC:2.1.4.1, AGAT, 1D12G5

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Product Information

68513-1-PBS targets GATM in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GATM fusion protein Ag3754
• Full Name: glycine amidinotransferase (L-arginine:glycine amidinotransferase)
• Calculated Molecular Weight: 423 aa, 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC004141
• Gene Symbol: GATM
• Gene ID (NCBI): 2628
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P50440
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

GATM, also known as AGAT, produces ornithine and guanidinoacetate (GAA) from arginine and glycine, and is a key enzyme for creatine synthesis. GATM deficiency in early infancy causes neurodevelopmental delay. GATM is predominantly expressed in kidney and pancreas in adults. Decrease of GAMT proteins was observed in proximal tubule damage cases, which links GATM as a general marker for proximal tubule damage. Alternative splicing of GATM generates three isoforms with molecular weights between 44 kDa to 48 kDa.