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GATM Monoclonal antibody

GATM Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1D12G5

Cat no : 68513-1-Ig

Synonyms

Transamidinase, L-arginine:glycine amidinotransferase, EC:2.1.4.1, AGAT, 1D12G5

Validation Data Gallery

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  • Western Blot (WB) analysis of pig kidney tissue using GATM Monoclonal antibody (68513-1-Ig)

    WB analysis of pig kidney using 68513-1-Ig

    pig kidney tissue were subjected to SDS PAGE followed by western blot with 68513-1-Ig (GATM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using GATM Monoclonal antibody (68513-1-Ig)

    WB analysis using 68513-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68513-1-Ig (GATM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inpig kidney tissue, rat kidney tissue, mouse kidney tissue, pig pancreas tissue, rat pancreas tissue, mouse pancreas tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68513-1-Ig targets GATM in WB, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GATM fusion protein Ag3754
Full Name glycine amidinotransferase (L-arginine:glycine amidinotransferase)
Calculated Molecular Weight 423 aa, 48 kDa
Observed Molecular Weight48 kDa
GenBank Accession NumberBC004141
Gene Symbol GATM
Gene ID (NCBI) 2628
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GATM, also known as AGAT, produces ornithine and guanidinoacetate (GAA) from arginine and glycine, and is a key enzyme for creatine synthesis. GATM deficiency in early infancy causes neurodevelopmental delay. GATM is predominantly expressed in kidney and pancreas in adults. Decrease of GAMT proteins was observed in proximal tubule damage cases, which links GATM as a general marker for proximal tubule damage. Alternative splicing of GATM generates three isoforms with molecular weights between 44 kDa to 48 kDa.

Protocols

Product Specific Protocols
WB protocol for GATM antibody 68513-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols