GATA4 Monoclonal antibody

GATA4 Monoclonal Antibody for WB, ELISA
Cat No. 68012-1-Ig
Clone No.2F5C6

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, ELISA

GATA binding factor 4, GATA binding protein 4, GATA4, Transcription factor GATA 4

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inPC-3 cells, HEK-293 cells, HepG2 cells, NCCIT cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68012-1-Ig targets GATA4 in WB, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GATA4 fusion protein Ag30392 Predict reactive species
Full Name GATA binding protein 4
Calculated Molecular Weight 442 aa, 45 kDa
Observed Molecular Weight44,45 kDa
GenBank Accession NumberBC101580
Gene Symbol GATA4
Gene ID (NCBI) 2626
RRIDAB_2918759
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP43694
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GATA4 is a transcriptional activator. GATA4 binds to the consensus sequence 5'-AGATAG-3'. GATA4 acts as a transcriptional activator of ANF in cooperation with NKX2-5. Mutation of GATA4 will cause the atrial septal defect type 2 (ASD2).

Protocols

Product Specific Protocols
WB protocol for GATA4 antibody 68012-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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