GATA4 Monoclonal antibody, PBS Only

GATA4 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F5C6

Cat no : 68012-1-PBS

Synonyms

GATA binding factor 4, GATA binding protein 4, GATA4, Transcription factor GATA 4

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Validation Data Gallery

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  WB analysis of PC-3 using 68012-1-Ig (same clone as 68012-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 68012-1-Ig (GATA4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68012-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 68012-1-Ig (same clone as 68012-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 68012-1-Ig (GATA4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68012-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 68012-1-Ig (same clone as 68012-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 68012-1-Ig (GATA4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68012-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NCCIT using 68012-1-Ig (same clone as 68012-1-PBS)

  NCCIT cells were subjected to SDS PAGE followed by western blot with 68012-1-Ig (GATA4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68012-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68012-1-PBS targets GATA4 in WB, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GATA4 fusion protein Ag30392
• Full Name: GATA binding protein 4
• Calculated Molecular Weight: 442 aa, 45 kDa
• Observed Molecular Weight: 44,45 kDa
• GenBank Accession Number: BC101580
• Gene Symbol: GATA4
• Gene ID (NCBI): 2626
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

GATA4 is a transcriptional activator. GATA4 binds to the consensus sequence 5'-AGATAG-3'. GATA4 acts as a transcriptional activator of ANF in cooperation with NKX2-5. Mutation of GATA4 will cause the atrial septal defect type 2 (ASD2).