CoraLite® Plus 488-conjugated GATA2 Polyclonal antibody

• 

  IF Staining of HepG2 using CL488-11103

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 GATA2 antibody (CL488-11103) at dilution of 1:200.

• Featured Product
• KD/KO Validated

GATA2 Polyclonal Antibody for IF

Cat No. CL488-11103

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Synonyms

FLJ45948, GATA binding protein 2, GATA2, NFE1B

---

Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-11103 targets GATA2 in IF applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GATA2 fusion protein Ag1557
• Full Name: GATA binding protein 2
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: BC018988
• Gene Symbol: GATA2
• Gene ID (NCBI): 2624
• ENSEMBL Gene ID: ENSG00000179348
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P23769
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

What is the molecular weight of GATA2? Is GATA2 post-translationally modified?

The molecular weight of GATA2 is 51 kDa. There are two known isoforms of GATA2 that use alternative first exons (PMID: 10731672). GATA2 can be phosphorylated, which regulates its activity (PMID: 7876160).

 What is the subcellular localization of GATA2?

GATA2 acts as a transcription factor in the nucleus. However, it can also be found in the cytoplasm (PMID: 25163535) and its phosphorylation promotes nuclear localization (PMID: 15837948).

 What is the tissue expression pattern of GATA2?

GATA2 was initially identified as a hematopoietic transcription factor regulating the differentiation of hematopoietic stem cells but is also present in early erythroid cells, mast cells, and megakaryocytes. Additionally, it is expressed in the central nervous system (PMID: 10357893).