GATA2 Polyclonal antibody

• Featured Product
• KD/KO Validated

GATA2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ChIP, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 11103-1-AP

Synonyms

NFE1B, GATA-binding protein 2, GATA binding protein 2, Endothelial transcription factor GATA-2

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Validation Data Gallery

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  WB analysis using 11103-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11103-1-AP (GATA2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 11103-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 11103-1-AP (GATA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 11103-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 11103-1-AP (GATA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11103-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11103-1-AP (GATA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 11103-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11103-1-AP (GATA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 11103-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11103-1-AP (GATA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11103-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11103-1-AP (GATA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 11103-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11103-1-AP (GATA2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11103-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11103-1-AP (GATA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 11103-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11103-1-AP (GATA2 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of SH-SY5Y using 11103-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using GATA2 antibody (11103-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, mouse liver tissue, K-562 cells, mouse heart tissue, rat heart tissue, HEK-293 cells, rat liver tissue
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• ChIP: See 2 publications below

Product Information

11103-1-AP targets GATA2 in WB, IHC, IF/ICC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GATA2 fusion protein Ag1557
• Full Name: GATA binding protein 2
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: BC018988
• Gene Symbol: GATA2
• Gene ID (NCBI): 2624
• RRID: AB_10914503
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

What is the molecular weight of GATA2? Is GATA2 post-translationally modified?

The molecular weight of GATA2 is 51 kDa. There are two known isoforms of GATA2 that use alternative first exons (PMID: 10731672). GATA2 can be phosphorylated, which regulates its activity (PMID: 7876160).

 What is the subcellular localization of GATA2?

GATA2 acts as a transcription factor in the nucleus. However, it can also be found in the cytoplasm (PMID: 25163535) and its phosphorylation promotes nuclear localization (PMID: 15837948).

 What is the tissue expression pattern of GATA2?

GATA2 was initially identified as a hematopoietic transcription factor regulating the differentiation of hematopoietic stem cells but is also present in early erythroid cells, mast cells, and megakaryocytes. Additionally, it is expressed in the central nervous system (PMID: 10357893).

Publications

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
human	IF	EMBO J; Functional screen reveals essential roles of miR-27a/24 in differentiation of embryonic stem cells.; Authors - Yanni Ma
human	WB	BMC Med; Downregulation of AC092894.1 promotes oxaliplatin resistance in colorectal cancer via the USP3/AR/RASGRP3 axis; Authors - Zhijian Zheng
human	WB	PLoS Genet; The PU.1-Modulated MicroRNA-22 Is a Regulator of Monocyte/Macrophage Differentiation and Acute Myeloid Leukemia.; Authors - Chao Shen
human	WB	Int J Mol Sci; LncRNA H19-Derived miR-675-5p Accelerates the Invasion of Extravillous Trophoblast Cells by Inhibiting GATA2 and Subsequently Activating Matrix Metalloproteinases.; Authors - Manabu Ogoyama
human	ChIP,IF	Endocrinology; PGE2 and Thrombin Induce Myofibroblast Transdifferentiation via Activin A and CTGF in Endometrial Stromal Cells.; Authors - Kazuya Kusama