GAS2L3 Polyclonal antibody

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  WB analysis using 25409-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25409-1-AP (GAS2L3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

GAS2L3 Polyclonal Antibody for WB, ELISA

Cat No. 25409-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

growth arrest 2 like 3, GAS2 like protein 3

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse heart tissue, rat heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25409-1-AP targets GAS2L3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GAS2L3 fusion protein Ag22097
• Full Name: growth arrest-specific 2 like 3
• Calculated Molecular Weight: 694 aa, 75 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC043366
• Gene Symbol: GAS2L3
• Gene ID (NCBI): 283431
• RRID: AB_3669473
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86XJ1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GAS2L3 also known as G2L3, belongs to the GAS2 family of four related proteins. It contains an N-terminal calponin homology (CH) domain and a C-terminal GAS2-related (GAR) domain, which are putative actin and microtubule binding domains. GAS2L3 is specifically expressed in mitosis and it localizes to the midbody and the constriction zone during cytokinesis (PMID: 28937871).