CoraLite® Plus 488-conjugated GARNL1 Recombinant antibody

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  FC experiment of U2OS using CL488-82971

  1x10^6 U2OS cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Garnl1 Recombinant Antibody (CL488-82971, Clone:230165F11)(red), or 0.4 ug CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

GARNL1 Recombinant Antibody for FC (Intra)

Cat No. CL488-82971

Clone No.230165F11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

Tuberin like protein 1, RALGAPA1, p240, KIAA0884, GRIPE

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Tested Applications

• Positive FC (Intra) detected in: U2OS cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-82971 targets GARNL1 in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GARNL1 fusion protein Ag31518
• Full Name: GTPase activating Rap/RanGAP domain-like 1
• Calculated Molecular Weight: 230KD
• Observed Molecular Weight: 260 kDa
• GenBank Accession Number: NM_014990.3
• Gene Symbol: GARNL1
• Gene ID (NCBI): 253959
• RRID: AB_3673158
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q6GYQ0
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GARNL1/RalGAPα1, a major α subunit of the Ral-GTPase activating protein in skeletal muscle, is a protein whose phosphorylation and binding to the regulatory 14-3-3 proteins is stimulated by insulin and also by muscle contraction (PMID:24768767).