GAMT Monoclonal antibody, PBS Only

GAMT Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat, mouse, pig

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E7B11

Cat no : 66322-1-PBS

Synonyms

GAMT, PIG2, TP53I2

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Validation Data Gallery

• 

  WB analysis of fetal human brain using 66322-1-Ig (same clone as 66322-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66322-1-Ig (GAMT Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66322-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 66322-1-Ig (same clone as 66322-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 66322-1-Ig (GAMT Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66322-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 66322-1-Ig (same clone as 66322-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66322-1-Ig (GAMT Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66322-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig liver using 66322-1-Ig (same clone as 66322-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66322-1-Ig (GAMT Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66322-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66322-1-PBS targets GAMT in WB, Indirect ELISA applications and shows reactivity with human, rat, mouse, pig samples.

• Tested Reactivity: human, rat, mouse, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: GAMT fusion protein Ag16778
• Full Name: guanidinoacetate N-methyltransferase
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC016760
• Gene Symbol: GAMT
• Gene ID (NCBI): 2593
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

GAMT converts the latter to creatine with S-adenosylmethionine as the methyl donor. GAMT was induced in human and mouse cells in response to genotoxic and metabolic stress. GAMT was required for p53-dependent upregulation of creatine biosynthesis, fatty acid oxidation, and apoptosis in response to glucose deprivation.