GADD45A Recombinant antibody

GADD45A Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241597H4

Cat no : 84376-4-RR

Synonyms

GADD45, DNA damage-inducible transcript 1 protein, DDIT-1, DDIT1, DDIT 1

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Validation Data Gallery

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  WB analysis using 84376-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84376-4-RR (GADD45A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of K562 using 84376-4-RR

  1x10^6 K562 were intracellularly stained with 0.25 ug GADD45A Recombinant antibody (84376-4-RR, Clone:241597H4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

• 

  FC experiment of U-2 OS using 84376-4-RR

  1x10^6 U-2 OS were intracellularly stained with 0.25 ug GADD45A Recombinant antibody (84376-4-RR, Clone:241597H4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

• 

  Affinity and Kinetic Characterization of 84376-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84376-4-RR against Human GADD45A were performed. The affinity constant is 0.769 nM.

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Tested Applications

• Positive WB detected in: K-562 cells, NIH/3T3 cells
• Positive FC (Intra) detected in: U-2 OS, K562

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84376-4-RR is GADD45A Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GADD45A fusion protein Ag4687
• Full Name: growth arrest and DNA-damage-inducible, alpha
• Calculated Molecular Weight: 165 aa, 18 kDa
• Observed Molecular Weight: 14-18 kDa, 28-36 kDa
• GenBank Accession Number: BC011757
• Gene Symbol: GADD45A
• Gene ID (NCBI): 1647
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GADD45A, also named as DDIT1 and GADD45, belongs to the GADD45 family. It binds to proliferating cell nuclear antigen. GADD45A may affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase. GADD45A plays an essential role in active DNA demethylation during terminal osteogenic differentiation of adipose-derived mesenchymal stem cells. GADD45A has Dimer (~28-36 kDa) and Monomer (14-18 kDa) forms (PMID:11498536).