GAD65 Polyclonal antibody, PBS Only

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  WB analysis using 20746-1-AP (same clone as 20746-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20746-1-AP (GAD65 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20746-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 20746-1-AP (same clone as 20746-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20746-1-AP (GAD65 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20746-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 20746-1-AP (same clone as 20746-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20746-1-AP (GAD65 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20746-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 20746-1-AP (same clone as 20746-1-PBS)

  IP result of anti-GAD65 (IP:20746-1-AP, 4ug; Detection:20746-1-AP 1:1000) with mouse brain tissue lysate 3440ug. This data was developed using the same antibody clone with 20746-1-PBS in a different storage buffer formulation.

GAD65 Polyclonal Antibody for WB, IP, Indirect ELISA

Cat No. 20746-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Synonyms

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Product Information

20746-1-PBS targets GAD65 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: NM_000818
• Gene Symbol: GAD65
• Gene ID (NCBI): 2572
• RRID: AB_10949196
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q05329
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

GAD2, also named as GAD65, belongs to the group II decarboxylase family. GAD2 catalyzes the production of GABA. It is responsible for the synthesis of the essential neurotransmitter gamma-aminobutyric acid (GABA) from L-glutamic acid. GAD2 is expressed in nervous and endocrine systems and are thought to be involved in synaptic transmission and INS secretion. Autoantibodies against GAD2 may serve as markers for type I diabetes. Many individuals suffering from an adult onset disorder known as Stiff Person Syndrome (SPS) also express autoantibodies to GAD2. The antibody is specific to GAD2.