GAD65 Polyclonal antibody

GAD65 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20746-1-AP

Synonyms

GAD 65, GAD2, GAD65, Glutamate decarboxylase 2



Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:3000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20746-1-AP targets GAD65 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
Calculated Molecular Weight 65 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberNM_000818
Gene Symbol GAD65
Gene ID (NCBI) 2572
RRIDAB_10949196
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GAD2, also named as GAD65, belongs to the group II decarboxylase family. GAD2 catalyzes the production of GABA. It is responsible for the synthesis of the essential neurotransmitter gamma-aminobutyric acid (GABA) from L-glutamic acid. GAD2 is expressed in nervous and endocrine systems and are thought to be involved in synaptic transmission and INS secretion. Autoantibodies against GAD2 may serve as markers for type I diabetes. Many individuals suffering from an adult onset disorder known as Stiff Person Syndrome (SPS) also express autoantibodies to GAD2. The antibody is specific to GAD2.

Protocols

Product Specific Protocols
WB protocol for GAD65 antibody 20746-1-APDownload protocol
IF protocol for GAD65 antibody 20746-1-APDownload protocol
IP protocol for GAD65 antibody 20746-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Nat Cell Biol

Cancer-cell-derived GABA promotes β-catenin-mediated tumour growth and immunosuppression.

Authors - De Huang
mouseWB

Neurobiol Stress

A sex-dependent delayed maturation of visual plasticity induced by adverse experiences in early childhood.

Authors - Yueqin Liu
mouseWB

Aging (Albany NY)

A five-m6A regulatory gene signature is a prognostic biomarker in lung adenocarcinoma patients.

Authors - Xiao Wu
mouseWB

Aging (Albany NY)

Maternal high sugar and fat diet benefits offspring brain function via targeting on the gut-brain axis.

Authors - Dongdong Wang
mouseWB

CNS Neurosci Ther

Integrin α3 is required for high-frequency repetitive transcranial magnetic stimulation-induced glutamatergic synaptic transmission in mice with ischemia

Authors - Li Liu
ratWB

Sci Rep

Renal Denervation Improves the Baroreflex and GABA System in Chronic Kidney Disease-induced Hypertension.

Authors - Hsin-Hung Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Lana (Verified Customer) (02-06-2020)

SDS-PAGE: 15 ug/ul RIPA lysate of whole striatal tissue. 4-12% Bis-tris gradient gel.Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4CBlocking: SEA Block Blocking Buffer 1hPrimary Ab: O/N incubation at 4C, 1:1000Secondary Ab: IRDye 800CW Goat anti-Rabbit, 1:15000.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Brain tissues, human.
GAD65 Antibody Western Blot, validation (1:1000 dilution) in Brain tissues, human. (Cat no:20746-1-AP)