Filter: AllWBIHCIF

WB analysis of pig brain using 67648-1-Ig (same clone as 67648-1-PBS)
pig brain tissue were subjected to SDS PAGE followed by western blot with 67648-1-Ig (GAD1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67648-1-PBS in a different storage buffer formulation.
WB analysis using 67648-1-Ig (same clone as 67648-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67648-1-Ig (GAD1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67648-1-PBS in a different storage buffer formulation.
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Various lysates were subjected to SDS PAGE followed by western blot with 67648-1-Ig (GAD1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67648-1-PBS in a different storage buffer formulation.
WB analysis using 67648-1-Ig (same clone as 67648-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67648-1-Ig (GAD1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67648-1-PBS in a different storage buffer formulation.
WB analysis of rat brain using 67648-1-Ig (same clone as 67648-1-PBS)
rat brain tissue were subjected to SDS PAGE followed by western blot with 67648-1-Ig (GAD1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67648-1-PBS in a different storage buffer formulation.
IHC staining of rat cerebellum using 67648-1-Ig (same clone as 67648-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67648-1-Ig (GAD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67648-1-PBS in a different storage buffer formulation.
IHC staining of mouse cerebellum using 67648-1-Ig (same clone as 67648-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67648-1-Ig (GAD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67648-1-PBS in a different storage buffer formulation.
IHC staining of mouse cerebellum using 67648-1-Ig (same clone as 67648-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67648-1-Ig (GAD1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67648-1-PBS in a different storage buffer formulation.
IHC staining of rat cerebellum using 67648-1-Ig (same clone as 67648-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67648-1-Ig (GAD1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67648-1-PBS in a different storage buffer formulation.
IF Staining of rat brain using 67648-1-Ig (same clone as 67648-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using GAD1 antibody (67648-1-Ig, Clone: 2D5B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67648-1-PBS in a different storage buffer formulation.

GAD1 Monoclonal antibody, PBS Only

GAD1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 67648-1-PBS

Clone No.2D5B7

Review Product

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

FLJ45882, GAD, GAD 67, GAD1, GAD67, Glutamate decarboxylase 1, SCP

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

67648-1-PBS targets GAD1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Reactivity	Human, mouse, rat, pig, rabbit
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	GAD1 fusion protein Ag21336 Predict reactive species
Full Name	glutamate decarboxylase 1 (brain, 67kDa)
Calculated Molecular Weight	67 kDa
Observed Molecular Weight	67 kDa, 57 kDa
GenBank Accession Number	BC002815
Gene Symbol	GAD1
Gene ID (NCBI)	2571
RRID	AB_2882848
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q99259
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

GAD1, also named as GAD67, belongs to the group II decarboxylase family. GAD1 is the rate-limiting enzyme responsible for γ-aminobutyric acid (GABA) biosynthesis from glutamatic acid and the major GAD isoform in the human brain for early brain development (PMID:21302352). It is also potentially involved in variety of skin activities.