GAD1 Monoclonal antibody
GAD1 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, mouse, rat, pig, rabbit and More (1)
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
CloneNo.
2D5B7
Cat no : 67648-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | pig brain tissue, rat brain tissue, mouse cerebellum tissue, pig cerebellum tissue, rabbit brain tissue, rabbit cerebellum tissue, rat cerebellum tissue, mouse brain tissue |
| Positive IHC detected in | rat cerebellum tissue, mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IF | See 3 publications below |
Product Information
67648-1-Ig targets GAD1 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.
| Tested Reactivity | Human, mouse, rat, pig, rabbit |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | GAD1 fusion protein Ag21336 |
| Full Name | glutamate decarboxylase 1 (brain, 67kDa) |
| Calculated Molecular Weight | 67 kDa |
| Observed Molecular Weight | 67 kDa, 57 kDa |
| GenBank Accession Number | BC002815 |
| Gene Symbol | GAD1 |
| Gene ID (NCBI) | 2571 |
| RRID | AB_2882848 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GAD1, also named as GAD67, belongs to the group II decarboxylase family. GAD1 is the rate-limiting enzyme responsible for γ-aminobutyric acid (GABA) biosynthesis from glutamatic acid and the major GAD isoform in the human brain for early brain development (PMID:21302352). It is also potentially involved in variety of skin activities.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GAD1 antibody 67648-1-Ig | Download protocol |
| IHC protocol for GAD1 antibody 67648-1-Ig | Download protocol |
| IF protocol for GAD1 antibody 67648-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Psychiatr Res NLRP3 in the GABAergic neuron induces cognitive impairments in a mouse model of hemorrhage shock and resuscitation | ||
Cells CREB1 Facilitates GABAergic Neural Differentiation of Human Mesenchymal Stem Cells through BRN2 for Pain Alleviation and Locomotion Recovery after Spinal Cord Injury | ||
Exp Neurol Hydrogen sulfide mitigates memory impairments via the restoration of glutamatergic neurons in a mouse model of hemorrhage shock and resuscitation |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Reyes (Verified Customer) (07-29-2024) | This is the first time a GAD antibody (in green) works properly in a human brain FFPE tissue.
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