GABRG2 Monoclonal antibody

GABRG2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H10E1

Cat no : 68595-1-Ig

Synonyms

GABAAR subunit gamma-2, GABA(A) receptor subunit gamma-2, GABA A receptor gamma 2, ECA2, CAE2

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Validation Data Gallery

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  WB analysis using 68595-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68595-1-Ig (GABRG2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: rabbit brain tissue, rat brain tissue, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68595-1-Ig targets GABRG2 in WB, ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.

• Tested Reactivity: human, mouse, rat, rabbit
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: GABRG2 fusion protein Ag5489
• Full Name: gamma-aminobutyric acid (GABA) A receptor, gamma 2
• Calculated Molecular Weight: 467 aa, 54 kDa
• Observed Molecular Weight: 40-54 kDa
• GenBank Accession Number: BC059389
• Gene Symbol: GABRG2
• Gene ID (NCBI): 2566
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GABRG2 (gamma-aminobutyric acid type A receptor subunit gamma2), which is expected to be located in cell membrane and expressed in brain and retina. The calculated molecular weight of the protein is 54 kDa. This antibody can recognize the 43 kDa band of target (PMID:12091434). And it also has glycosylation modification. GABA is the major inhibitory neurotransmitter in the mammlian brain, where it acts at GABA-A receptors, which are ligand-gated chloride channels. GABA-A receptors are pentameric, consisting of proteins from several subunit classes: alpha, beta, gamma, delta and rho. Mutations in this gene have been associated with epilepsy and febrile seizures.