Tested Applications
Positive WB detected in | unboiled rat brain tissue, unboiled mouse brain tissue, mouse cerebellum tissue |
Positive FC (Intra) detected in | A549 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:1000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL750-83057-2 targets GABRA2 in WB, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | GABRA2 fusion protein Ag29301 Predict reactive species |
Full Name | gamma-aminobutyric acid (GABA) A receptor, alpha 2 |
Calculated Molecular Weight | 51 kDa |
Observed Molecular Weight | 50-51 kDa |
GenBank Accession Number | NM_000807 |
Gene Symbol | GABRA2 |
Gene ID (NCBI) | 2555 |
RRID | AB_3673764 |
Conjugate | CoraLite® Plus 750 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 755 nm / 780 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P47869 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GABRA2 (gamma-aminobutyric acid type A receptor subunit alpha2), also known as DEE78. It is expected to be located in cell membrane, and the protein is highly expressed in brain. The protein is a ligand-gated chloride channel, which is a component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the brain (PubMed:29961870). It also plays an important role in the formation of functional inhibitory GABAergic synapses in addition to mediating synaptic inhibition as a GABA-gated ion channel (PubMed:29961870). The calculated molecular weight of GABRA2 is 51 kDa.
Protocols
Product Specific Protocols | |
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WB protocol for CL Plus 750 GABRA2 antibody CL750-83057-2 | Download protocol |
FC protocol for CL Plus 750 GABRA2 antibody CL750-83057-2 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |