CoraLite® Plus 750-conjugated GABRA2 Recombinant antibody

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  WB analysis using CL750-83057-2

  Various lysates were subjected to SDS PAGE followed by western blot with CL750-83057-2 (GABRA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  FC experiment of A549 using CL750-83057-2

  1x10^6 A549 cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated GABRA2 Recombinant antibody (CL750-83057-2, Clone:230158E6)(red), or 4 ug Isotype Control (CL750-30000, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

GABRA2 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra)

Cat No. CL750-83057-2

Clone No.230158E6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra)

Synonyms

230158E6

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Tested Applications

• Positive WB detected in: unboiled rat brain tissue, unboiled mouse brain tissue, mouse cerebellum tissue
• Positive FC (Intra) detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL750-83057-2 targets GABRA2 in WB, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GABRA2 fusion protein Ag29301
• Full Name: gamma-aminobutyric acid (GABA) A receptor, alpha 2
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 50-51 kDa
• GenBank Accession Number: NM_000807
• Gene Symbol: GABRA2
• Gene ID (NCBI): 2555
• RRID: AB_3673764
• Conjugate: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 755 nm / 780 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P47869
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GABRA2 (gamma-aminobutyric acid type A receptor subunit alpha2), also known as DEE78. It is expected to be located in cell membrane, and the protein is highly expressed in brain. The protein is a ligand-gated chloride channel, which is a component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the brain (PubMed:29961870). It also plays an important role in the formation of functional inhibitory GABAergic synapses in addition to mediating synaptic inhibition as a GABA-gated ion channel (PubMed:29961870). The calculated molecular weight of GABRA2 is 51 kDa.