GABPA Recombinant antibody, PBS Only

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  WB analysis using 84951-4-RR (same clone as 84951-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84951-4-RR (GABPA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84951-4-PBS in a different storage buffer formulation.

• 

  WB analysis using 84951-4-RR (same clone as 84951-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84951-4-RR (GABPA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84951-4-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 84951-4-RR (same clone as 84951-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 84951-4-RR (GABPA antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84951-4-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 84951-4-RR (same clone as 84951-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 84951-4-RR (GABPA antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84951-4-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 84951-4-RR (same clone as 84951-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using GABPA antibody (84951-4-RR, Clone: 242578F10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84951-4-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84951-4-RR (same clone as 84951-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GABPA antibody (84951-4-RR, Clone: 242578F10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84951-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84951-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84951-4-RR against Human GABPA were performed. The affinity constant is 2.51 nM.

GABPA {ptg:Brand} Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 84951-4-PBS

Clone No.242578F10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

242578F10, E4TF1 60, E4TF1A, GA binding protein alpha chain, GA-binding protein alpha chain

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Product Information

84951-4-PBS targets GABPA in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GABPA fusion protein Ag16191
• Full Name: GA binding protein transcription factor, alpha subunit 60kDa
• Calculated Molecular Weight: 454 aa, 51 kDa
• Observed Molecular Weight: 56-60 kDa
• GenBank Accession Number: BC035031
• Gene Symbol: GABPA
• Gene ID (NCBI): 2551
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q06546
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

GA-binding protein alpha chain (GABP alpha subunit, GABPA, nuclear respiratory factor 2 subunit alpha, transcription factor E4TF1-60) is one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit.GABPA is a member of Ets family, binds to the Yap promoter and activates YAP transcription(23684612). Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype.