Filter: AllWBIHCIF/ICCAffinity

WB analysis using 84951-4-RR
Various lysates were subjected to SDS PAGE followed by western blot with 84951-4-RR (GABPA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 84951-4-RR
Various lysates were subjected to SDS PAGE followed by western blot with 84951-4-RR (GABPA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC staining of human cervical cancer using 84951-4-RR
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 84951-4-RR (GABPA antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 84951-4-RR
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 84951-4-RR (GABPA antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 84951-4-RR
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GABPA antibody (84951-4-RR, Clone: 242578F10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
IF Staining of NIH/3T3 using 84951-4-RR
Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using GABPA antibody (84951-4-RR, Clone: 242578F10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
Affinity and Kinetic Characterization of 84951-4-RR
Biolayer interferometry (BLl) kinetic assays of 84951-4-RR against Human GABPA were performed. The affinity constant is 2.51 nM.

GABPA Recombinant antibody

GABPA {ptg:Brand} Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 84951-4-RR

Clone No.242578F10

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

242578F10, E4TF1 60, E4TF1A, GA binding protein alpha chain, GA-binding protein alpha chain

Formulation: PBS and Azide

PBS and Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HEK-293 cells, mouse brain tissue, K-562 cells, HuH-7 cells, HeLa cells, NIH/3T3 cells, rat brain tissue
Positive IHC detected in	human cervical cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	NIH/3T3 cells, HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84951-4-RR targets GABPA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	GABPA fusion protein Ag16191 Predict reactive species
Full Name	GA binding protein transcription factor, alpha subunit 60kDa
Calculated Molecular Weight	454 aa, 51 kDa
Observed Molecular Weight	56-60 kDa
GenBank Accession Number	BC035031
Gene Symbol	GABPA
Gene ID (NCBI)	2551
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purfication
UNIPROT ID	Q06546
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GA-binding protein alpha chain (GABP alpha subunit, GABPA, nuclear respiratory factor 2 subunit alpha, transcription factor E4TF1-60) is one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit.GABPA is a member of Ets family, binds to the Yap promoter and activates YAP transcription(23684612). Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype.

Protocols

Product Specific Protocols
WB protocol for GABPA antibody 84951-4-RR	Download protocol
IHC protocol for GABPA antibody 84951-4-RR	Download protocol
IF protocol for GABPA antibody 84951-4-RR	Download protocol