GABARAPL1 Monoclonal antibody, PBS Only

• 

  WB analysis of mouse brain using 66458-1-Ig (same clone as 66458-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66458-1-Ig (GABARAPL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66458-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 66458-1-Ig (same clone as 66458-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66458-1-Ig (GABARAPL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66458-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 66458-1-Ig (same clone as 66458-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66458-1-Ig (GABARAPL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66458-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66458-1-Ig (same clone as 66458-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GABARAPL1 antibody (66458-1-Ig, Clone: 1E10F6 ) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66458-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

GABARAPL1 Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 66458-1-PBS

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, ELISA

Synonyms

Early estrogen-regulated protein, ATG8L, ATG8, APG8L, APG8 LIKE

Formulation:  PBS Only

PBS and Azide

PBS Only

Conjugate:  Unconjugated

Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Product Information

66458-1-PBS targets GABARAPL1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GABARAPL1 fusion protein Ag1473
• Full Name: GABA(A) receptor-associated protein like 1
• Calculated Molecular Weight: 14 kDa
• Observed Molecular Weight: 16-18 kDa
• GenBank Accession Number: BC009309
• Gene Symbol: GABARAPL1
• Gene ID (NCBI): 23710
• RRID: AB_2881827
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9H0R8
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

GABARAPL1 (GABARAP-like protein 1), also named ATG8, GEC1, APG8L, ATG8L, and APG8-LIKE, is a member of the GABARP (GABAA receptor-associated protein) family. GABARAPL1 was initially identified as an estrogen-regulated gene and the protein acts in receptor and vesicle transport. It's also involved in the process of autophagy like GABARAP and GABARAPL2 and may be considered as an autophagic marker. It is expressed at very high levels in the brain, heart, peripheral blood leukocytes, liver, kidney, placenta, and skeletal muscle and at very low levels in the thymus and small intestine.