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  • Featured Product
  • KD/KO Validated

GABARAPL1 Monoclonal antibody

GABARAPL1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1E10F6

Cat no : 66458-1-Ig

Synonyms

APG8 LIKE, APG8L, ATG8, ATG8L, GABARAPL1, GEC 1, GEC1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using GABARAPL1 Monoclonal antibody (66458-1-Ig)

    WB analysis of mouse brain using 66458-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66458-1-Ig (GABARAPL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using GABARAPL1 Monoclonal antibody (66458-1-Ig)

    WB analysis of rat brain using 66458-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66458-1-Ig (GABARAPL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using GABARAPL1 Monoclonal antibody (66458-1-Ig)

    WB analysis of pig brain using 66458-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66458-1-Ig (GABARAPL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GABARAPL1 Monoclonal antibody (66458-1-Ig)

    IF Staining of HepG2 using 66458-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GABARAPL1 antibody (66458-1-Ig, Clone: 1E10F6 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue, pig brain tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66458-1-Ig targets GABARAPL1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GABARAPL1 fusion protein Ag1473
Full Name GABA(A) receptor-associated protein like 1
Calculated Molecular Weight 14 kDa
Observed Molecular Weight 16-18 kDa
GenBank Accession NumberBC009309
Gene Symbol GABARAPL1
Gene ID (NCBI) 23710
RRIDAB_2881827
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GABARAPL1 (GABARAP-like protein 1), also named as ATG8, GEC1, APG8L, ATG8L and APG8-LIKE, is a member of GABARP (GABAA receptor-associated protein) family. GABARAPL1 was initially identified as estrogen-regulated gene and the protein acts in receptor and vesicle transport. It's also involved in the process of autophagy like GABARAP and GABARAPL2, and may be considered as an autophagic marker. It is expressed at very high levels in the brain, heart, peripheral blood leukocytes, liver, kidney, placenta and skeletal muscle and at very low levels in thymus and small intestine.

Protocols

Product Specific Protocols
WB protocol for GABARAPL1 antibody 66458-1-IgDownload protocol
IF protocol for GABARAPL1 antibody 66458-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

PLoS Pathog

The mitophagy receptor NIX induces vIRF-1 oligomerization and interaction with GABARAPL1 for the promotion of HHV-8 reactivation-induced mitophagy

Authors - Mai Tram Vo
View Article
humanWB

Iran J Basic Med Sci

Targeting GABARAPL1/HIF-2a axis to induce tumor cell apoptosis in nasopharyngeal carcinoma

Authors - Xiaopeng Huang
  • KD Validated
View Article