G6PC Monoclonal antibody, PBS Only (Detector)

G6PC Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E11A5

Cat no : 66860-1-PBS

Synonyms

G6PC1, G6Pase-alpha, G-6-Pase, G6Pase, EC:3.1.3.9

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Validation Data Gallery

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  WB analysis using 66860-1-Ig (same clone as 66860-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66860-1-Ig (G6PC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66860-1-PBS in a different storage buffer formulation.

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  WB analysis of HuH-7 using 66860-1-Ig (same clone as 66860-1-PBS)

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 66860-1-Ig (G6PC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66860-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 66860-1-Ig (same clone as 66860-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 66860-1-Ig (G6PC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66860-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66860-1-Ig (same clone as 66860-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66860-1-Ig (G6PC antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66860-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66860-1-Ig (same clone as 66860-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66860-1-Ig (G6PC antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66860-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66860-1-Ig (same clone as 66860-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66860-1-Ig (G6PC antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66860-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66860-1-Ig (same clone as 66860-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66860-1-Ig (G6PC antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66860-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50826-2

  Cytometric bead array standard curve of MP50826-2, G6PC Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66860-4-PBS. Detection antibody: 66860-1-PBS. Standard:Ag17839. Range: 0.098-100 ng/mL.

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Product Information

66860-1-PBS targets G6PC as part of a matched antibody pair:

MP50826-2: 66860-4-PBS capture and 66860-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: G6PC fusion protein Ag17839
• Full Name: glucose-6-phosphatase, catalytic subunit
• Calculated Molecular Weight: 357 aa, 40 kDa
• Observed Molecular Weight: 37-42 kDa
• GenBank Accession Number: BC130478
• Gene Symbol: G6PC
• Gene ID (NCBI): 2538
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Glucose-6-phosphatase-α (G6PC) is a key enzyme in glucose homeostasis that catalyzes the hydrolysis of glucose-6-phosphate to glucose and phosphate in the terminal step of gluconeogenesis and glycogenolysis. G6PC activity is restricted to the liver , the kidney cortex and the small intestine and confers on these three organs the capacity to release glucose into the systemic circulation (PMID: 21983240).The encoded enzyme is anchored to the ER by nine transmembrane helices with the amino (N)-terminus in the lumen and the carboxyl (C)-terminus in the cytoplasm (PMID: 15542400).