G6PC Polyclonal antibody

G6PC Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29084-1-AP

Synonyms

G6PC1, G6PT, G6Pase-alpha, G-6-Pase, G6Pase

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Validation Data Gallery

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  WB analysis using 29084-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29084-1-AP (G6PC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HepG2 cells, L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29084-1-AP targets G6PC in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: G6PC fusion protein Ag30614
• Full Name: glucose-6-phosphatase, catalytic subunit
• Calculated Molecular Weight: 357 aa, 40 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC130478
• Gene Symbol: G6PC
• Gene ID (NCBI): 2538
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glucose-6-phosphatase-α (G6PC) is a key enzyme in glucose homeostasis that catalyzes the hydrolysis of glucose-6-phosphate to glucose and phosphate in the terminal step of gluconeogenesis and glycogenolysis. G6PC activity is restricted to the liver , the kidney cortex and the small intestine and confers on these three organs the capacity to release glucose into the systemic circulation (PMID: 21983240).The encoded enzyme is anchored to the ER by nine transmembrane helices with the amino (N)-terminus in the lumen and the carboxyl (C)-terminus in the cytoplasm (PMID: 15542400).