CoraLite®555-conjugated G3BP1 Polyclonal antibody

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  IF Staining of HeLa using CL555-13057

  Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using CoraLite®555 G3BP1 antibody (CL555-13057) at dilution of 1:400.

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  FC experiment of HeLa using CL555-13057

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human G3BP1 (CL555-13057) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

G3BP1 Polyclonal Antibody for IF/ICC, FC (Intra)

Cat No. CL555-13057

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

IF/ICC, FC (Intra)

Synonyms

G3BP, G3BP 1, G3BP1, HDH VIII

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Tested Applications

• Positive IF/ICC detected in: sodium arsenite treated HeLa cells
• Positive FC (Intra) detected in: HeLa cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL555-13057 targets G3BP1 in IF/ICC, FC (Intra) applications and shows reactivity with human, rat, mouse samples.

• Tested Reactivity: human, rat, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: G3BP1 fusion protein Ag3728
• Full Name: GTPase activating protein (SH3 domain) binding protein 1
• Calculated Molecular Weight: 466 aa, 52 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC006997
• Gene Symbol: G3BP1
• Gene ID (NCBI): 10146
• RRID: AB_2919614
• Conjugate: CoraLite®555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 557 nm / 570 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13283
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GAP SH3 Binding Protein 1 (G3BP1), also named as G3BP, is an effector of stress granule (SG) assembly. SG biology plays an important role in the pathophysiology of TDP-43 in ALS and FTLD-U. G3BP1 can be used as a marker of SG. It has been shown to function downstream of Ras and play a role in RNA metabolism, signal transduction, and proliferation. G3BP1 is a ubiquitously expressed protein that localizes to the cytoplasm in proliferating cells and to the nucleus in non-proliferating cells. G3BP1 has recently been implicated in cancer biology.