Tested Applications
Positive IF/ICC detected in | sodium arsenite treated HeLa cells |
Positive FC (Intra) detected in | HeLa cells |
Positive FC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL555-13057 targets G3BP1 in IF/ICC, FC (Intra) applications and shows reactivity with human, rat, mouse samples.
Tested Reactivity | human, rat, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | G3BP1 fusion protein Ag3728 Predict reactive species |
Full Name | GTPase activating protein (SH3 domain) binding protein 1 |
Calculated Molecular Weight | 466 aa, 52 kDa |
Observed Molecular Weight | 55-60 kDa |
GenBank Accession Number | BC006997 |
Gene Symbol | G3BP1 |
Gene ID (NCBI) | 10146 |
RRID | AB_2919614 |
Conjugate | CoraLite®555 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 557 nm / 570 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q13283 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GAP SH3 Binding Protein 1 (G3BP1), also named as G3BP, is an effector of stress granule (SG) assembly. SG biology plays an important role in the pathophysiology of TDP-43 in ALS and FTLD-U. G3BP1 can be used as a marker of SG. It has been shown to function downstream of Ras and play a role in RNA metabolism, signal transduction, and proliferation. G3BP1 is a ubiquitously expressed protein that localizes to the cytoplasm in proliferating cells and to the nucleus in non-proliferating cells. G3BP1 has recently been implicated in cancer biology.
Protocols
Product Specific Protocols | |
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IF protocol for CL555 G3BP1 antibody CL555-13057 | Download protocol |
FC protocol for CL555 G3BP1 antibody CL555-13057 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |