G3BP1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

G3BP1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E4A2

Cat no : 66486-1-PBS

Synonyms

G3BP, G3BP 1, G3BP1, HDH VIII

---

Validation Data Gallery

• 

  WB analysis using 66486-1-Ig (same clone as 66486-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66486-1-Ig (G3BP1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66486-1-Ig (same clone as 66486-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66486-1-Ig (G3BP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

• 

  WB analysis of RAW 264.7 using 66486-1-Ig (same clone as 66486-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66486-1-Ig (G3BP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 66486-1-Ig (same clone as 66486-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66486-1-Ig (G3BP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 66486-1-Ig (same clone as 66486-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66486-1-Ig (G3BP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 66486-1-Ig (same clone as 66486-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66486-1-Ig (G3BP antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 66486-1-Ig (same clone as 66486-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66486-1-Ig (G3BP antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 66486-1-Ig (same clone as 66486-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66486-1-Ig (G3BP1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 66486-1-Ig (same clone as 66486-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66486-1-Ig (G3BP1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 66486-1-Ig (same clone as 66486-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66486-1-Ig (G3BP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 66486-1-Ig (same clone as 66486-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66486-1-Ig (G3BP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 66486-1-Ig (same clone as 66486-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66486-1-Ig (G3BP antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 66486-1-Ig (same clone as 66486-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66486-1-Ig (G3BP antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66486-1-Ig (same clone as 66486-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using G3BP1 antibody (66486-1-Ig, Clone: 1E4A2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

---

Product Information

66486-1-PBS targets G3BP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: G3BP1 fusion protein Ag3728
• Full Name: GTPase activating protein (SH3 domain) binding protein 1
• Calculated Molecular Weight: 466 aa, 52 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC006997
• Gene Symbol: G3BP1
• Gene ID (NCBI): 10146
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

GAP SH3 Binding Protein 1 (G3BP1), also named as G3BP, is an effector of stress granule (SG) assembly. SG biology plays an important role in the pathophysiology of TDP-43 in ALS and FTLD-U. G3BP1 can be used as a marker of SG. It has been shown to function downstream of Ras and play a role in RNA metabolism, signal transduction, and proliferation. G3BP1 is a ubiquitously expressed protein that localizes to the cytoplasm in proliferating cells and to the nucleus in non-proliferating cells. G3BP1 has recently been implicated in cancer biology.