CoraLite®594-conjugated Follistatin Monoclonal antibody

Follistatin Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

4E9B10

Cat no : CL594-60060

Synonyms

Activin binding protein, follistatin, FS, FST

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Validation Data Gallery

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  IF Staining of HeLa using CL594-60060

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 Follistatin antibody (CL594-60060, Clone: 4E9B10 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60060 targets Follistatin in IF/ICC applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Follistatin fusion protein Ag0775
• Full Name: follistatin
• Calculated Molecular Weight: 33 aa, 7 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC004107
• Gene Symbol: Follistatin
• Gene ID (NCBI): 10468
• RRID: AB_2883413
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Caprylic Acid-Ammonium Sulfate Precipitation
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Follistatin (FST) is a member of the tissue growth factor β family and is a secreted glycoprotein that antagonizes many members of the family, including activin A, growth differentiation factor11 , and myostatin. It binds activin A with high affinity and whose expression can be induced by activin A and several other pro-inflammatory cytokines. Activin A-follistatin complexes are biologically inactive and bind to cell surface heparan sulphate-containing proteoglycans for internalisation and degradation. It has also been found to play a significant role in the management of skeletal muscle size and mass. It also has important roles in early embryonic development, differentiation of ovarian granulosa cells, liver fibrosis and polycystic ovarian syndrome.