Follistatin Polyclonal antibody, PBS Only

• 

  WB analysis using 27350-1-AP (same clone as 27350-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27350-1-AP (Follistatin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27350-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 27350-1-AP (same clone as 27350-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27350-1-AP (Follistatin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27350-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 27350-1-AP (same clone as 27350-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Follistatin antibody (27350-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27350-1-PBS in a different storage buffer formulation.

Follistatin Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 27350-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

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Product Information

27350-1-PBS targets Follistatin in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Follistatin fusion protein Ag26401
• Full Name: follistatin
• Calculated Molecular Weight: 33 aa, 7 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC004107
• Gene Symbol: Follistatin
• Gene ID (NCBI): 10468
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P19883
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Follistatin (FST) is a member of the tissue growth factor β family and is a secreted glycoprotein that antagonizes many members of the family, including activin A, growth differentiation factor11 , and myostatin. It binds activin A with high affinity and whose expression can be induced by activin A and several other pro-inflammatory cytokines. Activin A-follistatin complexes are biologically inactive and bind to cell surface heparan sulphate-containing proteoglycans for internalisation and degradation. It has also been found to play a significant role in the management of skeletal muscle size and mass. It also has important roles in early embryonic development, differentiation of ovarian granulosa cells, liver fibrosis and polycystic ovarian syndrome.