Follistatin Polyclonal antibody

Follistatin Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 27350-1-AP

Synonyms

Activin binding protein, follistatin, FS, FST

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Validation Data Gallery

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  WB analysis using 27350-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27350-1-AP (Follistatin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 27350-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27350-1-AP (Follistatin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 27350-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Follistatin antibody (27350-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, Calu-3 cells, HeLa cells, U2OS cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

27350-1-AP targets Follistatin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Follistatin fusion protein Ag26401
• Full Name: follistatin
• Calculated Molecular Weight: 33 aa, 7 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC004107
• Gene Symbol: Follistatin
• Gene ID (NCBI): 10468
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Follistatin (FST) is a member of the tissue growth factor β family and is a secreted glycoprotein that antagonizes many members of the family, including activin A, growth differentiation factor11 , and myostatin. It binds activin A with high affinity and whose expression can be induced by activin A and several other pro-inflammatory cytokines. Activin A-follistatin complexes are biologically inactive and bind to cell surface heparan sulphate-containing proteoglycans for internalisation and degradation. It has also been found to play a significant role in the management of skeletal muscle size and mass. It also has important roles in early embryonic development, differentiation of ovarian granulosa cells, liver fibrosis and polycystic ovarian syndrome.

Publications

Species	Application	Title
mouse,rat	IHC	Hypertens Res; Follistatin lowers blood pressure and improves vascular structure and function in essential and secondary hypertension; Authors - Ann Kuganathan