FlexAble HRP Antibody Labeling Kit for Rabbit IgG
Cat no : KFA005
Validation Data Gallery
Product Information
Product name | FlexAble HRP Antibody Labeling Kit for Rabbit IgG |
Assay type | Antibody labeling |
Tested applications | IF, IHC, WB |
Species Reactivity | Rabbit IgG |
Antibody amount per labeling reaction | 0.5 µg antibody |
Conjugate | HRP |
Compatible with | HRP reactive substrates and tyramide reagents |
Kit Components
| Component | 10 rxns | 50 rxns | 4×50 rxns |
| HRP FlexLinker for Rabbit IgG | 10 μL | 50 μL | 4×50 μL |
| FlexQuencher for Rabbit IgG | 20 μL | 100 μL | 4×100 μL |
| FlexBuffer | 100 μL | 500 μL | 4×500 μL |
Storage Condition
Store for 1 year at -20°C or for 6 months at +4°C after shipment. Avoid exposure to light.FAQ
Q: What are the FlexLinker, FlexQuencher and FlexBuffer?
A: The FlexLinker is a small polypeptide to which dyes are covalently conjugated that can label unconjugated primary antibodies. The FlexQuencher is an Fc-containing fragment that neutralizes the excess FlexLinker. The FlexBuffer is a PBS-based buffer.
Q: What is the largest quantity I can label?
A: With a standard kit size (50 reactions), you can label 25 µg of one antibody or up to 50 different antibodies. You can easily scale up the antibody amount per labeling approach.
Q: What is the lowest concentration of my primary antibody that I can use?
A: Our protocol uses 0.5 µg of primary antibody in 7 µL, which ends up at 0.07 mg/mL. If the concentration of your antibody is lower, you can also use a larger volume than 7 µL.
Q: Can I label primary antibodies stored in BSA, glycerol, Tris buffer and/or preservatives?
A: Yes, FlexAble Antibody Labeling Kits have been validated with carriers and amine buffers. Neither BSA nor amine buffers, in any chosen concentration, interfere with the labeling. 50% glycerol as well as preservatives like sodium azide are also compatible with the kit.
Q: How many different primary antibodies can I label with one kit?
A: You can label up to 50 different antibodies with our FlexAble 50 rxn Kit, and up to 10 antibodies with our FlexAble 10 rxn Kit.
Q: Will I observe cross-reactivity/leaking when I use two FlexAble-labeled antibodies from the same species during multiplexing?
A: FlexAble labels primary antibodies with a high affinity FlexLinker. Dissociation of FlexLinker from one antibody and association to another antibody is rare. If you observe leaking, we recommend adding more FlexQuencher to remove unbound FlexLinker, or you can try sequential staining of the labeled antibodies.
Publications
| Application | Title |
|---|---|
Front Immunol Prognostic biomarker DARS2 correlated with immune infiltrates in bladder tumor |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Tabea (Verified Customer) (06-24-2024) | I only tried it once with different concentrations for the Jess, and I managed to see a signal. There is still room for improvement, but that would require more assay dev, which I haven't done yet.
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