Product Information
| Product name | FlexAble HRP Antibody Labeling Kit for Mouse IgG1 |
| Assay type | Antibody labeling |
| Tested applications | IF, IHC, WB |
| Species Reactivity | Mouse IgG1 |
| Antibody amount per labeling reaction | 0.5 µg antibody |
| Conjugate | HRP |
| Compatible with | HRP reactive substrates and tyramide reagents |
Kit Components
| Component | 10 rxns | 50 rxns | 200rxns |
| FlexAble HRP Antibody Labeling Kit for Mouse IgG1 | 50rxns | ||
| FlexBuffer | 100ul | 500ul | |
| FlexLinker for Mouse IgG1 HRP | 10ul | 50ul | |
| FlexQuencher for Mouse IgG1 | 20ul | 100ul |
Storage Condition
Store for 1 year at -20°C or for 6 months at +4°C upon receipt. Avoid exposure to lightFAQ
Q: What are the FlexLinker, FlexQuencher and FlexBuffer?
A: The FlexLinker is a small polypeptide to which dyes are covalently conjugated that can label unconjugated primary antibodies. The FlexQuencher is an Fc-containing fragment that neutralizes the excess FlexLinker. The FlexBuffer is a PBS-based buffer.
Q: What is the largest quantity I can label?
A: With a standard kit size (50 reactions), you can label 25 µg of one antibody or up to 50 different antibodies. You can easily scale up the antibody amount per labeling approach.
Q: What is the lowest concentration of my primary antibody that I can use?
A: Our protocol uses 0.5 µg of primary antibody in 7 µL, which ends up at 0.07 mg/mL. If the concentration of your antibody is lower, you can also use a larger volume than 7 µL.
Q: Can I label primary antibodies stored in BSA, glycerol, Tris buffer and/or preservatives?
A: Yes, FlexAble Antibody Labeling Kits have been validated with carriers and amine buffers. Neither BSA nor amine buffers, in any chosen concentration, interfere with the labeling. 50% glycerol as well as preservatives like sodium azide are also compatible with the kit.
Q: How many different primary antibodies can I label with one kit?
A: You can label up to 50 different antibodies with our FlexAble 50 rxn Kit, and up to 10 antibodies with our FlexAble 10 rxn Kit.
Q: Will I observe cross-reactivity/leaking when I use two FlexAble-labeled antibodies from the same species during multiplexing?
A: FlexAble labels primary antibodies with a high affinity FlexLinker. Dissociation of FlexLinker from one antibody and association to another antibody is rare. If you observe leaking, we recommend adding more FlexQuencher to remove unbound FlexLinker, or you can try sequential staining of the labeled antibodies.
Publications
| Application | Title |
|---|---|
Front Immunol Prognostic biomarker DARS2 correlated with immune infiltrates in bladder tumor | |
Int J Biol Macromol Polyethyleneimine-modified Laminarin nanoparticles as a novel vaccine adjuvant for ovalbumin to enhance the immune responses |
