FlexAble CoraLite® Plus 488 Antibody Labeling Kit for Rabbit IgG
Cat no : KFA001
Validation Data Gallery
Product Information
FlexAble CoraLite® Plus 488 Antibody Labeling Kit for Rabbit IgG is a novel antibody labeling kit that uses an affinity linker to conjugate CoraLite® Plus 488 in any buffer condition to rabbit IgG primary antibodies from any supplier. One labeling reaction requires only 0.5µg of antibody – regardless of the antibody concentration. Label your rabbit IgG antibody in <10 min without any additional equipment.
Product name | FlexAble CoraLite® Plus 488 Antibody Labeling Kit for Rabbit IgG |
Assay type | Antibody labeling |
Tested applications | IF, FC, WB |
Species Reactivity | Rabbit IgG |
Antibody amount per labeling reaction | 0.5 µg antibody |
Conjugate | CoraLite® Plus 488 |
Excitation / Emission maxima wavelengths | 500 nm / 520 nm |
Kit Components
| Component | 10 rxns | 50 rxns | 4×50 rxns |
| CoraLite® Plus 488 FlexLinker for Rabbit IgG | 10 μL | 50 μL | 4×50 μL |
| FlexQuencher for Rabbit IgG | 20 μL | 100 μL | 4×100 μL |
| FlexBuffer | 100 μL | 500 μL | 4×500 μL |
Storage Condition
Store for 1 year at -20°C or for 6 months at +4°C after shipment. Avoid exposure to light.FAQ
Q: What are the FlexLinker, FlexQuencher and FlexBuffer?
A: The FlexLinker is a small polypeptide to which dyes are covalently conjugated that can label unconjugated primary antibodies. The FlexQuencher is an Fc-containing fragment that neutralizes the excess FlexLinker. The FlexBuffer is a PBS-based buffer.
Q: What is the largest quantity I can label?
A: With a standard kit size (50 reactions), you can label 25 µg of one antibody or up to 50 different antibodies. You can easily scale up the antibody amount per labeling approach.
Q: What is the lowest concentration of my primary antibody that I can use?
A: Our protocol uses 0.5 µg of primary antibody in 7 µL, which ends up at 0.07 mg/mL. If the concentration of your antibody is lower, you can also use a larger volume than 7 µL.
Q: Can I label primary antibodies stored in BSA, glycerol, Tris buffer and/or preservatives?
A: Yes, FlexAble Antibody Labeling Kits have been validated with carriers and amine buffers. Neither BSA nor amine buffers, in any chosen concentration, interfere with the labeling. 50% glycerol as well as preservatives like sodium azide are also compatible with the kit.
Q: How many different primary antibodies can I label with one kit?
A: You can label up to 50 different antibodies with our FlexAble 50 rxn Kit, and up to 10 antibodies with our FlexAble 10 rxn Kit.
Q: Will I observe cross-reactivity/leaking when I use two FlexAble-labeled antibodies from the same species during multiplexing?
A: FlexAble labels primary antibodies with a high affinity FlexLinker. Dissociation of FlexLinker from one antibody and association to another antibody is rare. If you observe leaking, we recommend adding more FlexQuencher to remove unbound FlexLinker, or you can try sequential staining of the labeled antibodies.
Publications
| Application | Title |
|---|---|
Stem Cell Res Ther A mutant methionyl-tRNA synthetase-based toolkit to assess induced-mesenchymal stromal cell secretome in mixed-culture disease models | |
J Cell Sci Iterative Bleaching Extends Multiplexity (IBEX) facilitates simultaneous identification of all major retinal cell types | |
Res Sq A Mutant Methionyl-tRNA Synthetase-Based toolkit to assess induced-Mesenchymal Stromal Cell secretome in mixed-culture disease models | |
Elife Interaction between Teneurin-2 and microtubules via EB proteins provides a platform for GABAA receptor exocytosis | |
Adv Sci (Weinh) BCAT2 Shapes a Noninflamed Tumor Microenvironment and Induces Resistance to Anti-PD-1/PD-L1 Immunotherapy by Negatively Regulating Proinflammatory Chemokines and Anticancer Immunity | |
Front Cell Neurosci K+/Cl- cotransporter 2 (KCC2) and Na+/HCO3- cotransporter 1 (NBCe1) interaction modulates profile of KCC2 phosphorylation |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Ramakrushna (Verified Customer) (09-02-2024) | Surprisingly works well with home made antibodies in yeast as well. The background is probably slightly higher than using primary followed by secondary antibody staining. But it does not affect in my case since I already know the localization of my protein.
|
FH Anita (Verified Customer) (04-08-2024) | All FlexAble Coral Lite fluorophores work very well with antibodies from different sources (companies).
|
FH Ruchi (Verified Customer) (06-02-2023) | The kit helped in saving the time and reduced the background. The attached pic of murine diabetic wounded skin stained via both direct and indirect method for Fibronectin. For the direct method Flexable CoraLite488 Rabbit IgG was used.
 |