DYKDDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope)
DYKDDDDK tag Monoclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
recombinant protein and More (5)
Applications
WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA
Conjugate
Unconjugated
199
CloneNo.
2B3C4
Cat no : 66008-3-Ig
Synonyms
"DYKDDDDK tag Antibodies" Comparison
View side-by-side comparison of DYKDDDDK tag antibodies from other vendors to find the one that best suits your research needs.
Tested Applications
| Positive WB detected in | Transfected HEK-293 cells |
| Positive IP detected in | Transfected HEK-293 cells |
| Positive IF/ICC detected in | Transfected HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66008-3-Ig targets DYKDDDDK tag in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with recombinant protein samples.
| Tested Reactivity | recombinant protein |
| Cited Reactivity | human, mouse, pig, canine, duck |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | DYKDDDDK tag |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2749837 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DYKDDDDK Tag ( Equivalent To FLAG Antibody From Sigma ) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DKYDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DYKDDDDK tag antibody 66008-3-Ig | Download protocol |
| IF protocol for DYKDDDDK tag antibody 66008-3-Ig | Download protocol |
| IP protocol for DYKDDDDK tag antibody 66008-3-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Science Structural insight into the SAM-mediated assembly of the mitochondrial TOM core complex. | ||
Nat Biomed Eng Lentiviral delivery of co-packaged Cas9 mRNA and a Vegfa-targeting guide RNA prevents wet age-related macular degeneration in mice. | ||
Nat Commun DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation | ||
Nat Commun ID1 expressing macrophages support cancer cell stemness and limit CD8+ T cell infiltration in colorectal cancer | ||
Mol Cell SMURF2 predisposes cancer cell toward ferroptosis in GPX4-independent manners by promoting GSTP1 degradation |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Yaman (Verified Customer) (02-14-2022) | This antibody was useful to my western blot experiments and also as negative control for most of my immunoprecipitation experiments.
 |
FH Christine (Verified Customer) (11-10-2021) | Very clean detection of overexpressed FLAG tagged protein in HEK293T cells.
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FH Jinwei (Verified Customer) (09-20-2021) | DYKDDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope) (66008-3-Ig) works fine.
 |
FH Chun (Verified Customer) (09-07-2020) | This is an excellent antibody for both IF and IB.
|
FH Chun (Verified Customer) (12-05-2019) | Excellent antibody
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FH David (Verified Customer) (08-07-2019) | Bacterial Expression system with Flag tag. This seems to be very similar to the other mouse flag tag that we bought before. This works great and we got good results.
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FH Daniela (Verified Customer) (06-03-2019) | In the figure, immunoblot of total cell lysate of HeLa cells transfected (left lane) with recombinant DNA expressing for a protein with the DDDDK-tag at its C-terminus. In the right lane, total cell lysate of HeLa moc transfection. Per each lane, 30ug of protein were loaded.
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