DDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope)

DDDDK tag Monoclonal Antibody for ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IF, IP, RIP, ELISA

Conjugate

Unconjugated

Publications

65

CloneNo.

2C3F4

Reviews (1)

Cat no : 66008-1-Ig

Synonyms

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• Validation Data Gallery
• Proteintech vs. Other Vendors

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  IP experiment of Transfected HEK-293 using 66008-1-Ig

  IP result of anti-DDDDK tag (IP:66008-1-Ig, 3ug; Detection:66008-1-Ig 1:10000) with Transfected HEK-293 cells lysate 300ug.

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  IF Staining of Transfected HEK-293 using 66008-1-Ig

  Immunofluorescent analysis of Transfected HEK-293 cells using 66008-1-Ig (Flag tag antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG (H+L).

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Published Applications

• WB: See 31 publications below
• IF: See 7 publications below
• IP: See 9 publications below
• RIP: See 1 publications below

Product Information

66008-1-Ig targets DDDDK tag in WB, IF, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: DDDDK tag
• RRID: AB_2881491
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DDDDK tag, also known as DDK tag, is a hydrophilic tag for recombinant protein technology with the sequence DYKDDDDK. The structure of DDDDK tag has been optimized for compatibility with the proteins it is attached to and unlikely to denature or inactivated them. For its high hydrophilic nature, the DDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag more likely to be accessible to antibodies. This product is generated against 1xDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DDDDK tags, no matter it is/they are located at N-terminal, C-terminal or at internal regions. The binding of this product with DDDDK tagged fusion protein does not depend on calcium ion. The clone of this monoclonal antibody hybridoma is 2C3F4, which is different from any other vendors.

Publications

Species	Application	Title
		Nat Microbiol; Ephrin receptor A2 is an epithelial cell receptor for Epstein-Barr virus entry.; Authors - Hua Zhang
	WB	Cell Metab; Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans.; Authors - Xiao Wang
	WB	Neuron; UBQLN2-HSP70 axis reduces poly-Gly-Ala aggregates and alleviates behavioral defects in the C9ORF72 animal model.; Authors - Kejing Zhang
	WB	Plant Cell; Structural Insight into DNA Recognition by CCT/NF-YB/YC Complexes in Plant Photoperiodic Flowering.; Authors - Cuicui Shen
human	WB	Cell Death Dis; The E3 ligase TRIM26 suppresses ferroptosis through catalyzing K63-linked ubiquitination of GPX4 in glioma; Authors - Zhangjie Wang
	WB	Mol Ther; Caspase-8 Induces Lysosome-Associated Cell Death in Cancer Cells.; Authors - Benfu Zhong

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Chun (Verified Customer) (07-03-2019)

This antibody is excellent

• Applications: Western Blot,
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: HEK293 and HeLa cells, and primary rat cortical neurons