Fibulin 5 Polyclonal antibody, PBS Only

Fibulin 5 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 29992-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 29992-1-AP (same clone as 29992-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29992-1-AP (Fibulin 5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29992-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 29992-1-AP (same clone as 29992-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29992-1-AP (Fibulin 5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29992-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 29992-1-AP (same clone as 29992-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29992-1-AP (Fibulin 5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29992-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 29992-1-AP (same clone as 29992-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 29992-1-AP (Fibulin 5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29992-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 29992-1-AP (same clone as 29992-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 29992-1-AP (Fibulin 5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29992-1-PBS in a different storage buffer formulation.

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Product Information

29992-1-PBS targets Fibulin 5 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Fibulin 5 fusion protein Ag30835
• Full Name: fibulin 5
• Calculated Molecular Weight: 448 aa, 50 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC022280
• Gene Symbol: Fibulin 5
• Gene ID (NCBI): 10516
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Fibulin-5, also known as EVEC and DANCE, is an extracellular matrix glycoprotein, secreted by various cell types such as vascular smooth muscle cells, fibroblasts and endothelial cells. Structurally, Fibulin-5 contains an Arg-Gly-Asp (RGD) motif and calcium-binding EGF-like domains. It promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. FBLN5 mutations have been described in patients with age-related macular degeneration, as well as being involved in Charcot-Marie-Tooth neuropathies.