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Fibulin 5 Polyclonal antibody

Fibulin 5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29992-1-AP

Synonyms

ARMD3, DANCE, EVEC, FBLN5, FIBL 5, fibulin 5, FLJ90059, UP50, Urine p50 protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Fibulin 5 Polyclonal antibody (29992-1-AP)

    WB analysis using 29992-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29992-1-AP (Fibulin 5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Fibulin 5 Polyclonal antibody (29992-1-AP)

    IHC staining of human lung cancer using 29992-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29992-1-AP (Fibulin 5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Fibulin 5 Polyclonal antibody (29992-1-AP)

    IHC staining of human colon cancer using 29992-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29992-1-AP (Fibulin 5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using Fibulin 5 Polyclonal antibody (29992-1-AP)

    IHC staining of mouse lung using 29992-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 29992-1-AP (Fibulin 5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using Fibulin 5 Polyclonal antibody (29992-1-AP)

    IHC staining of mouse colon using 29992-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 29992-1-AP (Fibulin 5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse heart tissue, mouse kidney tissue, rat heart tissue
Positive IHC detected inhuman colon cancer tissue, mouse lung tissue, mouse colon tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29992-1-AP targets Fibulin 5 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Fibulin 5 fusion protein Ag30835
Full Name fibulin 5
Calculated Molecular Weight 448 aa, 50 kDa
Observed Molecular Weight65-70 kDa
GenBank Accession NumberBC022280
Gene Symbol Fibulin 5
Gene ID (NCBI) 10516
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fibulin-5, also known as EVEC and DANCE, is an extracellular matrix glycoprotein, secreted by various cell types such as vascular smooth muscle cells, fibroblasts and endothelial cells. Structurally, Fibulin-5 contains an Arg-Gly-Asp (RGD) motif and calcium-binding EGF-like domains. It promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. FBLN5 mutations have been described in patients with age-related macular degeneration, as well as being involved in Charcot-Marie-Tooth neuropathies.

Protocols

Product Specific Protocols
WB protocol for Fibulin 5 antibody 29992-1-APDownload protocol
IHC protocol for Fibulin 5 antibody 29992-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols