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Fibrinogen Alpha Chain Polyclonal antibody

Fibrinogen Alpha Chain Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20645-1-AP

Synonyms

FGA, Fib2, fibrinogen alpha chain



Tested Applications

Positive WB detected inmouse liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20645-1-AP is Fibrinogen Alpha Chain Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Fibrinogen Alpha Chain fusion protein Ag9567
Full Name fibrinogen alpha chain
Calculated Molecular Weight 866 aa, 95 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC020764
Gene Symbol FGA
Gene ID (NCBI) 2243
RRIDAB_2878715
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fibrinogen is a soluble plasma glycoprotein synthesized in the liver. It is composed of two sets of three structurally different subunits: alpha (FGA), beta (FGB), gamma (FGG). Fibrinogen is converted by thrombin into fibrin during blood coagulation. Fibrinogen and fibrin play overlapping roles in blood clotting, fibrinolysis, cellular and matrix interactions, the inflammatory response, wound healing, and neoplasia (PMID: 16102057). FGA is the alpha chain of fibrinogen. During conversion of fibrinogen to fibrin, fibrinopeptide A is cleaved from FGA by thrombin.

Protocols

Product Specific Protocols
WB protocol for Fibrinogen Alpha Chain antibody 20645-1-APDownload protocol
IHC protocol for Fibrinogen Alpha Chain antibody 20645-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Nat Commun

Controllable gelation of artificial extracellular matrix for altering mass transport and improving cancer therapies.

Authors - Di-Wei Zheng
humanWB,IP

Free Radic Biol Med

SIRT5-mediated SDHA desuccinylation promotes clear cell renal cell carcinoma tumorigenesis.

Authors - Yuanzhen Ma
humanWB

Aging (Albany NY)

Serum proteomic predicts effectiveness and reveals potential biomarkers for complications in liver transplant patients.

Authors - Wenjing Wang
mouseWB

J Cell Mol Med

Over-expression of Dyrk1A affects bleeding by modulating plasma fibronectin and fibrinogen level in mice

Authors - Guillaume Postic
mouse

J Thromb Haemost

Chronic liver injury drives non-traditional intrahepatic fibrin(ogen) cross-linking via tissue transglutaminase.

Authors - L G Poole
humanIF

Phytomedicine

Geniposide alleviates VEGF-induced angiogenesis by inhibiting VEGFR2/PKC/ERK1/2-mediated SphK1 translocation.

Authors - Yan Wang