Fibrinogen Beta Chain Monoclonal antibody, PBS Only

Fibrinogen Beta Chain Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F8E4

Cat no : 66186-1-PBS

Synonyms

FGB, fibrinogen beta chain

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Validation Data Gallery

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  WB analysis of human heart using 66186-1-Ig (same clone as 66186-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66186-1-Ig (Fibrinogen beta chain antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66186-1-PBS in a different storage buffer formulation.

• 

  WB analysis of fetal human brain using 66186-1-Ig (same clone as 66186-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66186-1-Ig (Fibrinogen beta chain antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66186-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66186-1-PBS targets Fibrinogen Beta Chain in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Fibrinogen Beta Chain fusion protein Ag10205
• Full Name: fibrinogen beta chain
• Calculated Molecular Weight: 491 aa, 56 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC107766
• Gene Symbol: FGB
• Gene ID (NCBI): 2244
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Fibrinogen is a soluble plasma glycoprotein synthesized in the liver. It is composed of two sets of three structurally different subunits: alpha (FGA), beta (FGB), gamma (FGG). Fibrinogen is converted by thrombin into fibrin during blood coagulation. Fibrinogen and fibrin play overlapping roles in blood clotting, fibrinolysis, cellular and matrix interactions, the inflammatory response, wound healing, and neoplasia (PMID: 16102057). FGB is the beta chain of fibrinogen. During conversion of fibrinogen to fibrin, fibrinopeptide B is cleaved from FGB by thrombin. Mutations in the gene of FGB lead to several disorders, including afibrinogenemia, dysfibrinogenemia, hypodysfibrinogenemia and thrombotic tendency.