Fibrinogen Alpha Chain Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

Fibrinogen Alpha Chain Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 20645-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of mouse liver using 20645-1-AP (same clone as 20645-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20645-1-AP (Fibrinogen alpha chain antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20645-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 20645-1-AP (same clone as 20645-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20645-1-AP (Fibrinogen Alpha Chain antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20645-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 20645-1-AP (same clone as 20645-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20645-1-AP (Fibrinogen Alpha Chain antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20645-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 20645-1-AP (same clone as 20645-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20645-1-AP (Fibrinogen Alpha Chain antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20645-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 20645-1-AP (same clone as 20645-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20645-1-AP (Fibrinogen Alpha Chain antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20645-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 20645-1-AP (same clone as 20645-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20645-1-AP (Fibrinogen Alpha Chain antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20645-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 20645-1-AP (same clone as 20645-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20645-1-AP (Fibrinogen Alpha Chain antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20645-1-PBS in a different storage buffer formulation.

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Product Information

20645-1-PBS targets Fibrinogen Alpha Chain in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Fibrinogen Alpha Chain fusion protein Ag9567
• Full Name: fibrinogen alpha chain
• Calculated Molecular Weight: 866 aa, 95 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC020764
• Gene Symbol: Fibrinogen Alpha Chain
• Gene ID (NCBI): 2243
• RRID: AB_2878715
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Fibrinogen is a soluble plasma glycoprotein synthesized in the liver. It is composed of two sets of three structurally different subunits: alpha (FGA), beta (FGB), gamma (FGG). Fibrinogen is converted by thrombin into fibrin during blood coagulation. Fibrinogen and fibrin play overlapping roles in blood clotting, fibrinolysis, cellular and matrix interactions, the inflammatory response, wound healing, and neoplasia (PMID: 16102057). FGA is the alpha chain of fibrinogen. During conversion of fibrinogen to fibrin, fibrinopeptide A is cleaved from FGA by thrombin.