Fibrillin 1 Recombinant antibody

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  WB analysis of HeLa using 83836-4-RR

  HeLa cells were subjected to SDS PAGE followed by western blot with 83836-4-RR (Fibrillin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 83836-4-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83836-4-RR (Fibrillin 1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

• 

  Affinity and Kinetic Characterization of 83836-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83836-4-RR against Human Fibrillin 1 were performed. The affinity constant is 51.3 nM.

Fibrillin 1 Recombinant Antibody for WB, IHC, ELISA

Cat No. 83836-4-RR

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

FBN1, MASS, Fibrillin-1, FBN, Asprosin

Formulation:  PBS and Azide

PBS and Azide

PBS Only

Conjugate:  Unconjugated

Unconjugated

Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83836-4-RR targets Fibrillin 1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fibrillin 1 fusion protein Ag30283
• Full Name: fibrillin 1
• Observed Molecular Weight: 350 kDa
• GenBank Accession Number: BC146854
• Gene Symbol: Fibrillin 1
• Gene ID (NCBI): 2200
• RRID: AB_3671419
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P35555
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fibrillin-1 belongs to the fibrillin family. Fibrillins are structural components of 10-12 nm extracellular calcium-binding microfibrils, which occur either in association with elastin or in elastin-free bundles. Fibrillin-1-containing microfibrils provide long-term force bearing structural support. Defects in Fibrillin-1 are a cause of Marfan syndrome (MFS), isolated ectopia lentis (EL), Weill-Marchesani syndrome autosomal dominant (ADWMS), Shprintzen-Goldberg craniosynostosis syndrome (SGS) or MASS syndrome.