Fetuin-A/AHSG Recombinant antibody

Fetuin-A/AHSG Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1E4

Cat no : 80793-1-RR

Synonyms

AHSG, Fetuin-A, Alpha 2 Z globulin, alpha 2 HS glycoprotein, AHS

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Validation Data Gallery

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  WB analysis of human plasma using 80793-1-RR

  human plasma were subjected to SDS PAGE followed by western blot with 80793-1-RR (Fetuin-A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 80793-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80793-1-RR against Human AHSG were performed. The affinity constant is 5.52 nM.

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Tested Applications

• Positive WB detected in: human plasma, human heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

80793-1-RR targets Fetuin-A/AHSG in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fetuin-A/AHSG fusion protein Ag9853
• Full Name: alpha-2-HS-glycoprotein
• Calculated Molecular Weight: 367 aa, 39 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC048198
• Gene Symbol: Fetuin-A
• Gene ID (NCBI): 197
• RRID: AB_2918913
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fetuin-A, also named as Alpha2-HS glycoprotein (AHSG), is a member of cystatin superfamily of protease inhibitors. It is a highly expressed glycoprotein in various fetal tissues whereas it is mainly expressed by the liver in adults. Fetuin A, an extracellular inhibitor of transforming growth factor β, is a profibrogenic stimulus in liver disease. Circulating fetuin-A may be a beneficial serum biomarker in the detection of liver and vascular fibrosis progression in patients with non-alcoholic fatty liver disease. It is also involved in several functions, such as endocytosis, brain development and the formation of bone tissue. Fetuin-A exerts its effects on the cardiovascular system by two different mechanisms. On the one hand it inhibits ins signaling and induces ins resistance contributing to the onset of atherosclerosis and on the other hand it inhibits calcium deposition and protects from vascular calcification.