FceR1a Recombinant antibody

• 

  WB analysis using 84662-3-RR

  Human testis was subjected to SDS PAGE followed by western blot with 84662-3-RR (Fc epsilon RI alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

FceR1a Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 84662-3-RR

Clone No.241848D1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

FceRIa, FcERI, Fc-epsilon RI-alpha, FCE1A, Fc epsilon RI alpha

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Tested Applications

• Positive WB detected in: human testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84662-3-RR targets FceR1a in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide
• Calculated Molecular Weight: 30kDa
• Observed Molecular Weight: 55-65 kDa
• GenBank Accession Number: NM_001387280.1
• Gene Symbol: FceR1a
• Gene ID (NCBI): 2205
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P12319
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fc epsilon Receptor I alpha (FceR1a) is a single-pass type I membrane protein that contains 2 immunoglobulin-like domains. FceR1a forms a tetrameric complex, FceRI, with one beta and two gamma subunits (PMID: 19406478). FceRI is the high-affinity receptor for the Fc region of immunoglobulin E (IgE) (PMID: 1532373). It is expressed on mast cells and basophils and plays a central role in the allergic response (PMID: 2148316). The calculated molecular weight of FceR1a is 30 kDa, the 55- to 70-kDa bands detected by this antibody are probably caused by heterogeneous glycosylation (PMID: 11344350; 12671054).