CoraLite® Plus 488 Anti-Human Fas/CD95 Rabbit Recombinant Antibody

Fas/CD95 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240340E11

Cat no : CL488-98034

Synonyms

FAS, APT1, Apoptosis-mediating surface antigen FAS, Apo-1 antigen, APO-1

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Validation Data Gallery

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  FC experiment of human PBMCs using CL488-98034

  1x10^6 human PBMCs were surface stained with APC Anti-Human CD45RO, and 5 ul CoraLite® Plus 488 Anti-Human Fas/CD95 Rabbit Recombinant Antibody (CL488-98034, Clone:240340E11) (right), or Isotype Control (left). Cells were not fixed.

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  FC experiment of human PBMCs using CL488-98034

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human Fas/CD95 Rabbit Recombinant Antibody (CL488-98034, Clone:240340E11) (red), or Isotype Control (blue). Cells were not fixed.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-98034 is Fas/CD95 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: Fas (TNF receptor superfamily, member 6)
• Calculated Molecular Weight: 38 kDa
• GenBank Accession Number: NM_000043.6
• Gene Symbol: CD95
• Gene ID (NCBI): 355
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light.

Background Information

FAS, also named as CD95, APO-1, APT1, FAS1 and TNFRSF6, is a receptor for TNFSF6/FASLG. It is a cell surface receptor belonging to the TNF receptor superfamily, can mediate apoptosis by ligation with an agonistic anti-Fas antibody or Fas ligand. Stimulation of Fas results in the aggregation of its intracellular death domains, leading to the formation of the death-inducing signaling complex (DISC). FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both.