Anti-Mouse Fas/CD95 Rabbit Recombinant Antibody

Fas/CD95 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

241229C11

Cat no : 98135-1-RR

Synonyms

Fas, CD95, TNFR6, APT1, APO 1

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Validation Data Gallery

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  FC experiment of mouse thymocytes using 98135-1-RR

  1x10^6 mouse thymocytes were surface stained with 0.25 ug Anti-Mouse Fas/CD95 Rabbit Recombinant Antibody (98135-1-RR, Clone: 241229C11) or Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Mouse CD8a (53-6.7) (APC-65069, Clone: 53-6.7). Cells were not fixed.

• 

  FC experiment of mouse thymocytes using 98135-1-RR

  1x10^6 mouse thymocytes were surface stained with 0.25 ug Anti-Mouse Fas/CD95 Rabbit Recombinant Antibody (98135-1-RR, Clone: 241229C11) (red) or Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse thymocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98135-1-RR targets Fas/CD95 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FusionProtein
• Full Name: Fas (TNF receptor superfamily member 6)
• Calculated Molecular Weight: 37kDa
• GenBank Accession Number: NM_007987.2
• Gene Symbol: Fas/CD95
• Gene ID (NCBI): 14102
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Fas (CD95/APO-1) is a transmembrane glycoprotein belonging to the tumor necrosis factor (TNF) receptor superfamily. It can mediate apoptosis by ligation with an agonistic anti-Fas antibody or Fas ligand. Stimulation of Fas results in the aggregation of its intracellular death domains, leading to the formation of the death-inducing signaling complex (DISC). FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The molecular mass of native Fas is 38 kDa, the high molecular weight form (40-55 kDa) of Fas is due to glycosylation.