Anti-Human Fas/CD95 Rabbit Recombinant Antibody, PBS Only

Fas/CD95 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240340G11

Cat no : 98034-2-PBS

Synonyms

FAS, APT1, Apoptosis-mediating surface antigen FAS, Apo-1 antigen, APO-1

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Validation Data Gallery

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  FC experiment of human PBMCs using 98034-2-RR (same clone as 98034-2-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human Fas/CD95 Rabbit Recombinant Antibody (98034-2-RR, Clone: 240340G11) or Isotype Control and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD45RO (UCHL1) (CL647-65150, Clone: UCHL1). Cells were not fixed. This data was developed using the same antibody clone with 98034-2-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98034-2-RR (same clone as 98034-2-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human Fas/CD95 Rabbit Recombinant Antibody (98034-2-RR, Clone: 240340G11) (red) or Isotype Control (blue), and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98034-2-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 98034-2-RR

  Biolayer interferometry (BLl) kinetic assays of 98034-2-RR against Human Fas/CD95 were performed. The affinity constant is 0.191 nM.

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Product Information

The immunogen of 98034-2-PBS is Fas/CD95 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: Fas (TNF receptor superfamily, member 6)
• Calculated Molecular Weight: 38 kDa
• GenBank Accession Number: NM_000043.6
• Gene Symbol: Fas/CD95
• Gene ID (NCBI): 355
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

FAS, also named as CD95, APO-1, APT1, FAS1 and TNFRSF6, is a receptor for TNFSF6/FASLG. It is a cell surface receptor belonging to the TNF receptor superfamily, can mediate apoptosis by ligation with an agonistic anti-Fas antibody or Fas ligand. Stimulation of Fas results in the aggregation of its intracellular death domains, leading to the formation of the death-inducing signaling complex (DISC). FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both.