Fas/CD95 Polyclonal antibody, PBS Only

• 

  WB analysis of Jurkat using 13098-1-AP (same clone as 13098-1-PBS)

  WB result of FAS/CD95 antibody (13098-1-AP, 1:1000) with si-control and si-FAS transfected Jurkat cells. This data was developed using the same antibody clone with 13098-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 13098-1-AP (same clone as 13098-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13098-1-AP (FAS/CD95 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13098-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 13098-1-AP (same clone as 13098-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13098-1-AP (FAS/CD95 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13098-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 13098-1-AP (same clone as 13098-1-PBS)

  IP result of anti-FAS/CD95 (IP:13098-1-AP, 3ug; Detection:13098-1-AP 1:500) with HeLa cells lysate 3000ug. This data was developed using the same antibody clone with 13098-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

Fas/CD95 Polyclonal Antibody for WB, IP, Indirect ELISA

Cat No. 13098-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, Indirect ELISA

Synonyms

---

Product Information

13098-1-PBS targets Fas/CD95 in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Fas/CD95 fusion protein Ag3718
• Full Name: Fas (TNF receptor superfamily, member 6)
• Calculated Molecular Weight: 35-38 kDa
• Observed Molecular Weight: 38-45 kDa
• GenBank Accession Number: BC012479
• Gene Symbol: Fas/CD95
• Gene ID (NCBI): 355
• RRID: AB_2278042
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P25445
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Fas (CD95/APO-1) is a transmembrane glycoprotein belonging to the tumor necrosis factor (TNF) receptor superfamily. It can mediate apoptosis by ligation with an agonistic anti-Fas antibody or Fas ligand. Stimulation of Fas results in the aggregation of its intracellular death domains, leading to the formation of the death-inducing signaling complex (DISC). FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The molecular mass of native Fas is 38 kDa, the high molecular weight form (40-55 kDa) of Fas is due to glycosylation.