Filter: AllWBIHCFC (Intra)

WB analysis using 66325-1-Ig (same clone as 66325-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66325-1-Ig (Factor XIIIa antibody) at dilution of 1:20000 and incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66325-1-PBS in a different storage buffer formulation.
WB analysis of human peripheral blood platelets using 66325-1-Ig (same clone as 66325-1-PBS)
human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 66325-1-Ig (Factor XIIIa antibody) at dilution of 1:20000 and incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66325-1-PBS in a different storage buffer formulation.
WB analysis of rabbit large intestine using 66325-1-Ig (same clone as 66325-1-PBS)
rabbit large intestine tissue was subjected to SDS PAGE followed by western blot with 66325-1-Ig (Factor XIIIa antibody) at dilution of 1:20000 and incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66325-1-PBS in a different storage buffer formulation.
WB analysis of human placenta using 66325-1-Ig (same clone as 66325-1-PBS)
human placenta tissue were subjected to SDS PAGE followed by western blot with 66325-1-Ig (Factor XIIIA Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66325-1-PBS in a different storage buffer formulation.
WB analysis of rat colon using 66325-1-Ig (same clone as 66325-1-PBS)
rat colon tissue was subjected to SDS PAGE followed by western blot with 66325-1-Ig (Factor XIIIa antibody) at dilution of 1:20000 and incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66325-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 66325-1-Ig (same clone as 66325-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66325-1-Ig (Factor XIIIA antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66325-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 66325-1-Ig (same clone as 66325-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66325-1-Ig (Factor XIIIA antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66325-1-PBS in a different storage buffer formulation.
FC experiment of U-937 using 66325-1-Ig (same clone as 66325-1-PBS)
1x10^6 U-937 cells were intracellularly stained with 0.25 ug Factor XIIIa Monoclonal antibody (66325-1-Ig, Clone:2D2B2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66325-1-PBS in a different storage buffer formulation.
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1x10^6 U-937 cells were intracellularly stained with 0.25 ug Factor XIIIa Monoclonal antibody (66325-1-Ig, Clone:2D2B2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66325-1-PBS in a different storage buffer formulation.

Factor XIIIa Monoclonal antibody, PBS Only

Factor XIIIa Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 66325-1-PBS

Clone No.2D2B2

Review Product

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, Indirect ELISA

Synonyms

F13A1, Protein-glutamine gamma-glutamyltransferase A chain, F13A, EC:2.3.2.13, Coagulation factor XIIIa

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

66325-1-PBS targets Factor XIIIa in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity	human, mouse, rat, pig, rabbit
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	Factor XIIIa fusion protein Ag11105 Predict reactive species
Full Name	coagulation factor XIII, A1 polypeptide
Calculated Molecular Weight	732 aa, 83 kDa
Observed Molecular Weight	75-80 kDa
GenBank Accession Number	BC027963
Gene Symbol	Factor XIIIa
Gene ID (NCBI)	2162
RRID	AB_2881706
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P00488
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

Factor XIIIa is a blood proenzyme that catalyzes intermolecular cross-linking of fibrinogen and is involved in clotting cascade. It has been identified in various tissues including placenta, uterus, and prostate. Anti- Factor XIIIa has been found to be useful in differentiating between dermatofibroma (90% positive) and desmoplastic malignant melanoma (negative). Factor XIIIa positivity is also seen in capillary hemagioblastoma, hemangioendothelioma, hemangiopericytoma, xanthogranuloma, xanthoma, hepatocellular carcinoma, glomus tumor , and meningioma.