Factor XIIIa Monoclonal antibody, PBS Only

Factor XIIIa Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig, mouse, rat, rabbit

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D2B2

Cat no : 66325-1-PBS

Synonyms

Coagulation factor XIIIa, F13A, F13A1, Factor XIIIA, Transglutaminase A chain

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Validation Data Gallery

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  WB analysis using 66325-1-Ig (same clone as 66325-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66325-1-Ig (Factor XIIIa antibody) at dilution of 1:20000 and incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66325-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human peripheral blood platelets using 66325-1-Ig (same clone as 66325-1-PBS)

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 66325-1-Ig (Factor XIIIa antibody) at dilution of 1:20000 and incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66325-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rabbit large intestine using 66325-1-Ig (same clone as 66325-1-PBS)

  rabbit large intestine tissue was subjected to SDS PAGE followed by western blot with 66325-1-Ig (Factor XIIIa antibody) at dilution of 1:20000 and incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66325-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 66325-1-Ig (same clone as 66325-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66325-1-Ig (Factor XIIIA Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66325-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat colon using 66325-1-Ig (same clone as 66325-1-PBS)

  rat colon tissue was subjected to SDS PAGE followed by western blot with 66325-1-Ig (Factor XIIIa antibody) at dilution of 1:20000 and incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66325-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66325-1-Ig (same clone as 66325-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66325-1-Ig (Factor XIIIA antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66325-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66325-1-Ig (same clone as 66325-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66325-1-Ig (Factor XIIIA antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66325-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66325-1-PBS targets Factor XIIIa in WB, IHC, Indirect ELISA applications and shows reactivity with human, pig, mouse, rat, rabbit samples.

• Tested Reactivity: human, pig, mouse, rat, rabbit
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Factor XIIIa fusion protein Ag11105
• Full Name: coagulation factor XIII, A1 polypeptide
• Calculated Molecular Weight: 732 aa, 83 kDa
• Observed Molecular Weight: 75-80 kDa
• GenBank Accession Number: BC027963
• Gene Symbol: F13A1
• Gene ID (NCBI): 2162
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Factor XIIIa is a blood proenzyme that catalyzes intermolecular cross-linking of fibrinogen and is involved in clotting cascade. It has been identified in various tissues including placenta, uterus, and prostate. Anti- Factor XIIIa has been found to be useful in differentiating between dermatofibroma (90% positive) and desmoplastic malignant melanoma (negative). Factor XIIIa positivity is also seen in capillary hemagioblastoma, hemangioendothelioma, hemangiopericytoma, xanthogranuloma, xanthoma, hepatocellular carcinoma, glomus tumor , and meningioma.